Manipulating the mouse embryo
by Marina Gertsenstein , Kristina Vintersten , Richard Behringer
No reviews yet
First published: 2002
1 language
ISBN: 9781936113019
Description
Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.
